HPLC is a laboratory technique used to separate, identify, and quantify the components of a mixture. It's a type of liquid chromatography that uses high pressure to push the mobile phase (a solvent) through a stationary phase (a column packed with particles). *Basic Components of an HPLC System:* 1. *Injector*: Introduces the sample into the system.2. *Column*: Separates the components of the mixture based on their interactions with the stationary phase.3. *Mobile Phase*: A solvent that carries the sample through the column.4. *Detector*: Measures the presence and amount of each component.5. *Pump*: Delivers the mobile phase at high pressure. *Basic Principles of HPLC:* 1. *Partitioning*: Components of the mixture distribute between the mobile phase and the stationary phase based on their chemical properties.2. *Adsorption*: Components interact with the stationary phase through adsorption, which affects their retention time.3. *Size Exclusion*: Larger molecules are excluded from the po

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